C4SH01
Multi-Slide Holder Tray
MAX3SLH
Fixed Microscope Slide Holder
Application Idea
The LPSA1 slide holder with a microscope slide mounted on the LPS710 piezo stage.
SLH1
Microscope Slide Clips
XYF2
XY Translation Mount
with Spring Clips
XYF1
XY Translation Mount
with Setscrews
Fixed Microscope Slide Holders
Thorlabs offers two options for fixed microscope slide holders. The MAX3SLH fixed slide holder is compatible with microscope slides or test targets as well as any rectangular optic that has a minimum width of 1.73" (44.0 mm). It is directly compatible with our 3-axis flexure stages, as well as other translation stages that have 1/4"-20 (M6) taps on 2" (50.8 mm) centers. For mounting multiple microscope slides at once, we offer the C4SH01 slide holder, which supports up to four 25 mm x 75 mm, 1.1 ± 0.2 mm thick microscope slides per tray, enabling fast analysis of multiple cultures. It is directly compatible with any microscope stage insert that accepts standard multiwell microplates, such as Thorlabs’ Z-axis piezo stage or XY scanning stage (when the MLS203P1 plate adapter is also installed). The C4SH01 slide holder is not compatible with the 26 mm x 76 mm slides sold by Thorlabs.
Translating Microscope Slide Holders
We offer two one-axis slide holders, the XF50(/M) for 50 mm translation and the XF100(/M) for 100 mm translation, as well two two-axis mounts (Item #s XYF1(/M) and XYF2(/M)). Both two-axis mounts provide translation over a 50 mm x 30 mm area. All translating microscope mounts are designed to provide stable, self-contained translation. The XF50(/M), XF100(/M), and XYF1(/M) mounts are ideal for mounting our microscope slides, test targets, filters, or any rectangular optic up to 126.2 mm (5") wide and 0.16" (4 mm) thick, while the XYF2(/M) accepts microscope slides and other optics between 2.95" (75.0 mm) to 3.03" (77.0 mm) wide, 0.98" (25.0 mm) to 3.03" (77.0 mm) tall, and 0.04" (1.0 mm) to 0.07" (1.7 mm) thick. For indirect mounting solutions utilizing fixed filter or optic mounts, the XYA1(/M) two-axis translation adapter also provides translation over a 50 mm x 30 mm area. Additionally, we have available the LPS710E(/M) amplified piezo stage with the LPSA1(/M) microscope slide holder; when used together, this stage and slide holder provide long range, fine focus Z-axis travel for a microscope slide while maintaining a compact, low-profile footprint. The LPSA1(/M) slide holder is compatible with microscope slides and test targets, as well as any rectangular optic from 2.88" (73.1 mm) to 3.00" (76.5 mm) wide and up to 0.055" (1.4 mm) thick and 1.00" (25.4 mm) tall.
Microscope Slide Spring Clips
The SLH1(/M) microscope slide spring clips can be attached to any stage with 8-32 (M4) tapped holes to hold microscope slides or test targets of various sizes. Each clip is 2.51" (63.7 mm) long with a 0.20" (5.1 mm) wide tip and can be rotated 360° for easy access to slides. Three dowel pins are included to align slides over the central aperture of our XYR1(/M) and XYT1(/M) Translation Stages.
Vernier scales are typically used to add precision to standard, evenly divided scales (such as the scales on Thorlabs' rotation, goniometric, or translation mounts). A vernier scale has found common use in many precision measurement instruments, the most common being calipers and micrometers. The vernier scale uses two scales side-by-side: the main scale and the vernier scale. The direct vernier scale has a slightly smaller spacing between its tick marks owing to the vernier scale having N ticks for every N - 1 ticks on the main scale. Hence, the lines on the main scale will not line up with all the lines on the vernier scale. Only one line from the vernier scale will match well with one line of the main scale, and that is the trick to reading a vernier scale.
Figures 1 through 3 show a linear vernier scale system for three different situations. In each case, the scale on the left is the main scale, while the small scale on the right is the vernier scale. When reading a vernier scale, the main scale is used for the gross number, and the vernier scale gives the precision value. In this manner, a standard ruler or micrometer can become a precision instrument.
The 0 on the vernier scale is the "pointer" (marked by a red arrow in Figures 1 - 5) and will indicate the main scale reading. In Figure 1 we see the pointer is lined up directly with the 75.6 line. Notice that the only other vernier scale tick mark that lines up well with the main scale is 10. Since the pointer lines up with the main scale’s 75.6, the reading from Figure 1 is 75.60 (in whatever units the instrument measures).
That is essentially all there is to reading a vernier scale. It's a very straightforward way of increasing the precision of a measurement instrument. To expound, let’s look at Figure 2. Here we see that the pointer is no longer aligned with a line on the main scale, but instead it is slightly above 75.6 and below 75.7; thus, the gross measurement is 75.6. The first vernier line that coincides with a main scale line is the 5, shown with a blue arrow. The vernier scale gives the final digit of precision; since the 5 is aligned to the main scale, the precision measurement for Figure 2 is 75.65.
Since this vernier scale is 10% smaller than the main scale, moving the vernier scale by 1/10 of the main scale will align the next vernier marking. This asks the obvious question: what if the measurement is within the 1/10 precision of the vernier scale? Figure 3 shows just this. Again, the pointer line is in between 75.6 and 75.7, yielding the gross measurement of 75.6. If we look closely, we see that the vernier scale 7 (marked with a blue arrow) is very closely aligned to the main scale, giving a precision measurement of 75.67. However, the vernier scale 7 is very slightly above the main scale mark, and we can see that the vernier scale 8 (directly above 7) is slightly below its corresponding main scale mark. Hence, the scale on Figure 3 could be read as 75.673 ± 0.002. A reading error of about 0.002 would be appropriate for
this instrument.
The vernier scale may also be used on rotating scales where the main scale and vernier scale do not share units. Figures 4 and 5 show a vernier scale system for two different situations where the main scale is given in degrees and the vernier scale has ticks every 5 arcmin (60 arcmin = 1°). In each case, the scale on the top is the main scale, while the small scale on the bottom is the vernier scale.
In Figure 4 we see the pointer is lined up directly with the 341° line. Notice that the only other vernier scale tick marks that line up well with the main scale are ±60 arcmin. Since the pointer lines up with the main scale at 341°, the reading from Figure 4 is 341.00°.
There are two ways to determine the reading if the zero on the vernier scale line is between two lines of the main scale. For the first method, take the line on the left side of the pointer on the vernier scale and subtract that value (in arcmin) from the value on the main scale that is to the right on the main scale. As an example, in Figure 5 the vernier pointer is between 342° and 343°; using the left blue arrow of the vernier scale results in
As we've seen here, vernier scales add precision to a standard scale measurement. While it takes a bit of getting used to, with a little practice, reading these scales is fairly straightforward. Vernier scales, whether they are direct or retrograde*, are read in the same fashion.
*A retrograde vernier scale has a larger spacing between its tick marks with N ticks for every N + 1 ticks on the main scale.
The MAX3SLH Microscopy Slide Holder allows petri dishes and microscope slides to be mounted on motion control stages for integration into custom microscopy setups such as optical tweezers. Two sets of mounting holes allow versatility. One set of four #6 (M3) counterbores is compatible with our 3-axis NanoMax flexure stages. A set of 1/4" (M6) slots with 2" (50.8 mm) separation is designed for mounting to many other components, including those with a 2" imperial hole pattern.
The spring clips are rotatable to accommodate easy swapping of petri dishes and microscope slides. There is a 1" long clear aperture between the mounting surfaces of the clips. They are also interchangeable with the SLH1 Microscope Slide Spring Clips sold below, as they both use 8-32 screws to secure them.
Part Number | Description | Price | Availability |
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MAX3SLH | Customer Inspired! Microscopy Slide Holder | $147.91 | Today |
The C4SH01 Four-Position Microscope Slide Holder, made from black PEI plastic, allows up to four 25 mm x 75 mm, 1.1 ± 0.2 mm thick slides to be mounted on our microscope stages. Designed for inverted microscopes, this slide holder is highly versatile and adaptable for automated tissue analysis on multiple slides. As shown in the image to the right, slides are loaded into the bottom of the tray, ensuring excellent stability and maintaining near parfocality from slide to slide. This slide holder has the external footprint of a standard multiwell plate (127.6 mm × 85.5 mm), allowing it to be easily integrated into existing microscope stages (e.g., Thorlabs' MLS203 fitted with MLS203P1 or MZS500-E) or robotic plate loaders.
Note: The C4SH01 is not compatible with the 26 mm x 76 mm slides sold by Thorlabs.
Part Number | Description | Price | Availability |
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C4SH01 | Four-Position Microscope Slide Holder | $96.22 | Today |
Thorlabs' One-Axis Rectangular Optic Mounts accept rectangular optics up to 4.0 mm (0.16") thick. The XF50(/M) offers 50 mm of travel and can mount optics from 12.7 mm (0.50") to 76.2 mm (3.00") wide. The XF100(/M) offers 100 mm of travel and can mount optics from 12.7 mm (0.50") to 126.2 mm (4.97") wide. These mounts are designed for use with our selection of resolution, distortion, slant edge, and calibration test targets. In addition, they are also compatible with our rectangular microscope slides, filters, dichroic mirrors, variable ND filters, and fluorescence imaging filters.
The actuator on the side of each mount enables travel along a single axis. The actuator can be adjusted by hand or with a 5/64" (2.0 mm) hex key (not included). A vernier scale that provides a resolution of 100 µm allows for repeatable positioning of the mounted optic. The actuator can be locked by a side-located 5/64" (2.0 mm) hex setscrew. Seven 8-32 (M4) tapped holes support several possible mounting orientations when used with our Ø1/2" posts. The three tapped holes along the front face of the mount are through holes allowing a post to be mounted on either side. Three of the mounting faces feature tapped holes spaced so that two Ø1/2" posts in post holders can be used next to each other for added stability.
Optics are secured with two support arms that each contain two nylon-tipped setscrews with a 5/64" (2.0 mm) hex. Each support arm can slide along a channel in the mount to accommodate different optic sizes; a 5/64" (2.0 mm) hex setscrew is used to lock each support arm in place. This mounting mechanism can be seen in the photo to the upper right. By loosening the top locking screws, the arms can be removed from the channel and interchanged, as shown in the image to the bottom left. When reattaching the arms, simply align each locking screw with one of the 2-56 captive nuts in the channel and tighten. This feature is useful in applications that require the target to be positioned close to a lens or other optical element in a system.
Please note that the support arms overlap the optic by 4.4 mm on each side.
These Rectangular Optic Mounts can be used to position a rectangular optic horizontally, vertically, or perpendicular to the post axis and can be mounted using one or two posts.
Part Number | Description | Price | Availability |
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XF50/M | One-Axis 50 mm Translation Mount for 12.7 mm - 76.2 mm Rectangular Optics, M4 Taps | $310.95 | Today |
XF100/M | Customer Inspired! One-Axis 100 mm Translation Mount for 12.7 mm - 126.2 mm Rectangular Optics, M4 Taps | $333.56 | Today |
XF50 | One-Axis 50 mm Translation Mount for 12.7 mm - 76.2 mm Rectangular Optics, 8-32 Taps | $310.95 | Today |
XF100 | Customer Inspired! One-Axis 100 mm Translation Mount for 12.7 mm - 126.2 mm Rectangular Optics, 8-32 Taps | $333.56 | Today |
Thorlabs' XYF1(/M) Two-Axis Mount accepts a 1/2" (12.7 mm) to 3" (76.2 mm) wide rectangular optic up to 0.16" (4 mm) thick. The XYF2(/M) Two-Axis Mount accepts 2.95" (75.0 mm) to 3.03" (77.0 mm) wide, 0.98" (25.0 mm) to 3.03" (77.0 mm) tall, and 0.04" (1.0 mm) to 0.07" (1.7 mm) thick microscope slides and other optics. The mounts are ideal for general applications as well as home-built microscopy systems. The XYF1(/M) is designed for use with our selection of resolution, distortion, slant edge, and calibration test targets. In addition, it is also compatible with our rectangular microscope slides, filters, dichroic mirrors, variable ND filters, and fluorescence imaging filters. While the XYF2(/M) was designed for use with our microscope slides, it may also be used with a select range of resolution, distortion, and calibration test targets as well as filters and fluorescence imaging filters that do not exceed the size parameters. Alternatively, to accommodate other optic shapes and sizes, Thorlabs' XYA1(/M) translation adapter (available below) provides the same translation range as the XYF1(/M) and XYF2(/M) mounts, but features counterbores and threaded holes for mounting a fixed optic mount, such as a fixed filter holder.
Two actuator knobs on the side of each mount enable manual positioning over a 50 mm (1.97") x 30 mm (1.18") area, providing both vertical and horizontal translation. As an alternative to using the knobs, the actuators can also be adjusted using a 5/64" (2 mm) hex key or ball driver (not included). Next to each knob is a brass setscrew with a 5/64" (2 mm) hex for locking the translation axes, allowing for lockable, repeatable positioning of the mounted optic. Each mount is also equipped with a vernier scale that provides a resolution of 100 µm. Five 8-32 (M4) tapped holes support six possible mounting orientations when used with our Ø1/2" posts, of which a selection are shown below.
Optics are secured in the XYF1(/M) mount with two support arms that each contain two 5/64" (2 mm) hex, nylon-tipped setscrews. Each support arm can slide along a channel in the mount to accommodate different optic sizes; a 5/64" (2 mm) hex setscrew is used to lock each support arm in place. This mounting mechanism can be seen in the photo to the upper right. By loosening the top locking screws, the arms can be removed from the channel and reversed, as shown in the image to the bottom left. When reattaching the arms, simply align each locking screw with one of the 2-56 captive nuts in the channel and tighten. This feature is useful in applications that require the target to be positioned close to a lens or other optical element in a system. Please note that the support arms overlap the optic by 4.4 mm on each side.
Optics are secured in the XYF2(/M) mount using two fixed spring clips. Please note that the distance between the two cannot be adjusted. To install a microscope slide in the XYF2(/M) mount, gently press on the push bar, lifting up the spring clips, and slide the optic in (see photo to the right). A 1.3 mm (0.05") hex setscrew below each spring clip allows fine adjustment of the distance between it and the back plate.
The XYF2(/M) (left and middle) and XYF1(/M) (right) mounts can be used to position a rectangular optic
(Item # R1L3S6P) horizontally, vertically, or perpendicular to the post axis.
Part Number | Description | Price | Availability |
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XYF1/M | XY Mount for 1/2" - 3" Rectangular Optics, M4 Taps | $401.80 | Today |
XYF2/M | XY Mount for 75.0 - 77.0 mm Wide, 25.0 - 77.0 mm Tall, 1.0 - 1.7 mm Thick Rectangular Optics, M4 Taps | $450.00 | 3 Weeks |
XYF1 | XY Mount for 1/2" - 3" Rectangular Optics, 8-32 Taps | $401.80 | Today |
XYF2 | XY Mount for 75.0 - 77.0 mm Wide, 25.0 - 77.0 mm Tall, 1.0 - 1.7 mm Thick Rectangular Optics, 8-32 Taps | $450.00 | Today |
Thorlabs' XYA1(/M) Two-Axis Translation Adapter provides XY translation for fixed optic mounts (such as fixed lens, fixed mirror, and fixed filter mounts) and other small components that attach via an 8-32 (M4) tap or counterbore. The removable top bar features two 8-32 (M4 x 0.7) tapped holes and three #8 (M4) counterbored holes on the top, with six 8-32 (M4 x 0.7) tapped through holes on the sides. These features make the adapter ideal for general applications as well as home-built microscopy systems. For a version of this XY translation adapter that directly accepts rectangular optics, please see the XYF1(/M) Rectangular Optic Mount.
Two actuator knobs on the side of the mount enable manual positioning over a 50 mm (1.97") x 30 mm (1.18") area, which can be oriented either vertically or horizontally. These actuators can be adjusted by hand or with a 5/64" (2 mm) hex key or ball driver (not included). Next to each knob is a brass setscrew with a 5/64" (2 mm) hex for locking the traslation axes, allowing for lockable, repeatable positioning of the mounted component. This mount is also equipped with a vernier scale that provides a resolution of 100 µm; please see the Vernier Scales tab for more information. Five 8-32 (M4) tapped holes support several possible mounting orientations when used with our Ø1/2" posts, such that either translation axis can be oriented vertically or both axes can be oriented horizontally. A selection of possible mounting orientations is shown below.
The removable top bar is secured via two captive 5/64" (2 mm) hex setscrews which lock it in place. By loosening these locking screws, the top bar can be removed so that components can be mounted using the counterbored holes. To reattach the top bar, tip the adapter towards one end so that the 2-56 captive nuts in the top channel fall to one side. Replace the top bar and tighten the lower of the captive 5/64" (2 mm) hex setscrews. Then, tilt the adapter towards the other end and allow the second 2-56 captive nut to slide to that side of the adapter. The second locking screw can then be tightened, securing the top bar in place. This process can be seen in the image to the right.
The XYA1 Adapter can be used in conjunction with fixed filter and lens mounts to position test targets, dichroic beamsplitters, and other optics. Either translation axis can be oriented vertically, or they can both be oriented horizontally.
Part Number | Description | Price | Availability |
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XYA1/M | Customer Inspired! XY Translation Adapter, M4 Threads and M4 Counterbores | $408.00 | Today |
XYA1 | XY Translation Adapter, 8-32 Threads and #8 Counterbores | $408.00 | Today |
LPS710E(/M) Key Specificationsa | ||
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Drive Voltage Range (Max) | -25 V to 150 V | |
Travel | Open Loop | 1100 µm ± 10% |
Closed Loop | 800 µm | |
Resolution | Open Loop | 2 nm |
Closed Loop | 6 nm | |
Accuracy | Closed Loop | ±0.06% (Over Full Travel Range) |
Bidirectional Repeatability | Closed Loop | ±0.03% (Over Full Travel Range) |
Angular Error | Closed Loop | ±40 µrad (Over Full Travel Range) |
Maximum Loadb | 300 g (0.66 lbs) | |
Outer Dimensions (L x W x H) |
130 mm x 28 mm x 30 mm (5.1" x 1.1" x 1.2") |
The LPS710E(/M) Amplified Piezo Stage provides long range, fine focus Z-axis travel for a microscope slide while maintaining a compact, low-profile footprint. It features submicron repeatability, nanometer positioning resolution, low angular error, and fast millisecond response and settling times. Each stage is shipped with a piezo controller that has been factory calibrated to the specific stage to achieve maximum accuracy. When used with the LPSA1(/M) microscope slide holder, the stage is ideal for imaging modalities requiring sensitive sample positioning and sectioning, such as confocal laser scanning microscopy. This stage is not compatible with trans-illumination.
The stage is driven by a discrete piezo stack housed within a hardened steel flexure structure that preloads the piezo stack and amplifies its displacement. See the table to the right for key specifications. The base of the stage includes two slots for 1/4"-20 (M6) cap screws and two slots for 4-40 (M3) cap screws that can be used to secure the stage to a stage or breadboard for use with a microscope, such as our Rigid Stands or Translating Platforms, or directly to an optical table. In addition, the stage has two bottom- and side-located 4-40 (M3) mounting holes that can be used to secure the stage using our line of mini-series components.
The LPSA1(/M) slide holder uses two countersunk cap screws to attach to the 21.0 mm x 21.0 mm mounting surface on the top of the LPS710E(/M) stage. The mounting surface is equipped with four 4-40 (M3) blind mounting holes that are 4.8 mm deep to orient the slide holder in two orthogonal positions on the stage. The slide holder is compatible with microscope slides and test targets, as well as any rectangular optic from 2.88" (73.1 mm) to 3.00" (76.5 mm) wide and up to 0.055" (1.4 mm) thick and 1.00" (25.4 mm) tall. Slides are secured using the red, spring-loaded plunger, as shown in the image to the upper right.
More details on this piezo stage and microscope slide holder are available at their full web presentation.
Part Number | Description | Price | Availability |
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LPS710E/M | Piezo Stage and Paired Controller, 1100 µm Travel, M3 Mounting Taps | $5,415.54 | Today |
LPSA1/M | Microscope Slide Holder for Amplified Piezo Stage, Metric | $192.17 | Today |
LPS710E | Piezo Stage and Paired Controller, 1100 µm Travel, 4-40 Mounting Taps | $5,415.54 | Lead Time |
LPSA1 | Microscope Slide Holder for Amplified Piezo Stage, Imperial | $192.17 | Today |
These steel spring clips, which are ideal for securely holding various sizes of microscope slides and test targets, come with two 8-32 (M4) threaded shoulder screws, each of which has a 3/32" (2.5 mm) hex socket for simple attachment to a variety of stages. Each clip is 2.51" (63.7 mm) long with a 0.20" (5.1 mm) wide tip and can be rotated 360° for easy access to, or positioning of, a slide. The tip of the clip is curved upwards to help prevent damage and facilitate the mounting of slides, while also providing enough holding force to secure it in place.
The inclusion of three Ø1/8" x 1/4" dowel pins makes these spring clips an excellent choice for use with our XYR1(/M) and XYT1(/M) Translation Stages (see the image to the right). These stages have a central Ø1" hole that is SM1 threaded (1.035"-40), and the top plate contains three alignment holes that are used with the Ø1/8" dowel pins. The dowel pins are positioned on the stage so that standard 1" x 3" (25.4 mm x 76.2 mm) slides are centered over the aperture, allowing for repeatable and accurate positioning of the slides.
Please note that any XYR1(/M) stage purchased before June 4, 2014 does not have dowel pin holes in the top mounting plate.
Part Number | Description | Price | Availability |
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SLH1/M | Microscope Slide Spring Clips, M4 Thread, Qty: 2 | $50.18 | Today |
SLH1/M | Microscope Slide Spring Clips, M4 Thread, Qty: 2 | $50.18 | Today |
SLH1 | Microscope Slide Spring Clips, 8-32 Thread, Qty: 2 | $50.18 | Today |