Products HomeMiniaturized Two-Photon Microscope (Mini2P)
- Designed for Calcium Imaging in Freely Moving Specimens
- Interchangeable Objectives for Application-Specific Customization
- Two MEMS Laser Scanning Options
A Mini2P Mounted on a
Ø6 mm Optical Post
(Post Not Included)
Somatosensory Cortex Expressing GCaMP6
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cortex expressing GCaMP6 and was taken with the Thorlabs’ Mini2P Microscope.
(Sample preparation courtesy of Mario Fernandez de la Puebla. Imaging performed at Dr. Weijian Zong’s lab at Kavli Institute for Systems Neuroscience, Norway.)
**Large-Scale Two-Photon Calcium Imaging in Freely Moving Mice.
Zong, W., Obenhaus, H. A., Skytøen, E. R., Eneqvist, H., de Jong, N. L., ... & Moser, E. I.
Features
- Stable Two-Photon Imaging without Impediment to Specimen Behavior*
- Fast Multiplane Imaging with a Microtunable Lens (<0.4 ms Response Time)
- Simultaneous Measurement of Hundreds of Neurons is Possible
- On-Board Detection with a High-Sensitivity Silicon Photomultiplier (SiPM)
- Two MEMS Scanner Configurations:
- Fast Scanning: 0.17 mm2 FOV, 40 Hz (256 x 256 Pixels)
- Large FOV: 0.25 mm2 FOV, 15 Hz (256 x 256 Pixels)
- Multiple Interchangeable Objectives
Thorlabs' Miniature Two-Photon Imaging System (Mini2P) is a fast, high-resolution miniscope designed for in vivo two-photon imaging of non-stationary specimens. This Mini2P is based on the original design referenced in the paper by Zong. W., et al.** The lightweight, head-mounted design allows for stable calcium imaging without impediment to the sample. Interchangeable objectives facilitate use with cranial windows, prisms, and GRIN lenses for imaging tissue at different depths and orientations. The Mini2P allows for hundreds of neurons to be monitored in a single FOV, while visualization of up to a thousand cells is achievable through multiplane imaging controlled through the built-in microtunable lens.
A near-infrared, 920 nm femtosecond pulsed laser provides ideal excitation for two-photon imaging of GFP. Fiber coupling with a hollow-core photonic crystal fiber creates a stable system without complicated alignment procedures. Laser scanning is performed by a 2D microelectromechanical system (MEMS) device that is available for either fast scanning or large FOV applications. Signal detection is achieved with an on-board, dual-color silicon photomultiplier (SiPM) detector, eliminating the need for a fiber collection bundle that could impede movement. Laser scanning, signal detection, and z-scanning are all integrated into a flexible circuit controlled by a single miniaturized coax cable bundle and dedicated control box.
*The Mini2P microscope is under development and all specifications are subject to change.
Sam Tesfai
General Manager,
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A Mini2P Mounted to a Mini-Series Optical Post, connected to an MSH3 Swivel Base and MSWC Swivel Post Clamp (Post Assembly Not Included)
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| Specificationsa | ||
|---|---|---|
| Scanner | Large FOV | Fast Scanning |
| Imaging Speed | 15 Hz (256 x 256 Pixels) | 40 Hz (256 x 256 Pixels) |
| Detector | Silicon Photomultiplier (SiPM) | |
| Objective Threading | M5 x 0.5 | |
| Laser | ||
| Center Wavelength | 920 nm | |
| Pulse Width | 125 fs | |
| Objective Optionsa | |||
|---|---|---|---|
| Magnification | 3X | 3X | 7X |
| Collection NA | 0.50 | 0.45 | 0.70 |
| Working Distance | 0.582 mm (Air) | 0 - 0.7 mm (Air/Water) 1.5 - 2 mm (Glass) |
0.24 mm (Water) 0.17 mm (Glass) |
| Use Case | GRIN Lens | Prism | Window |
| Focus Rangeb | 240 µm | 240 µm | ~40 µm |
Thorlabs recognizes that each imaging application has unique requirements.
If you have any feedback, questions, or need a quotation, please
use our Contact Form or call (703) 651-1700.
| Posted Comments: | |
cdolbashian
(posted 2024-07-19 04:15:56.0) Thank you for reaching out to us with this quote request. We have contacted you directly regarding it. Lay Tin
(posted 2024-03-28 00:23:04.25) Hi,
Our customer is looking for 2P Microscope for Calcium Imaging in mouse. May we know how does your Miniaturized Two-Photon Microscope (Mini2P) work? Separately do you have laser for conventional Two-Photon Microscope?
Thanks and regards,
Laytin
Bronjo Medi
20 Woodlands Link
#03-08, Singapore 738733
fon: +65-6-4 76 40 11
fax: +65-6-7 53 73 03
email: bronjo@singnet.com.sg
web: www.bronjo.com jdelia
(posted 2024-03-28 11:06:24.0) Thank you for contacting Thorlabs. We have reached out to you directly to discuss your application. |
Miniature Two-Photon Microscope