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Assembled Mini2P Mounted to a Mini-Series Optical Post, connected to an MSH3 Swivel Base and MSWC Swivel Post Clamp (Post Assembly Not Included)

Sam Tesfai
General Manager,

Thorlabs Imaging Systems

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Features

• Stable Two-Photon Imaging without Impediment to Specimen Behavior*
• Fast Multiplane Imaging with a Microtunable Lens (<0.4 ms Response Time)
• Simultaneous Measurement of Hundreds of Neurons is Possible 
• On-Board Detection with a High-Sensitivity Silicon Photomultiplier (SiPM)
• Two MEMS Scanner Configurations:
  • Fast Scanning: 0.17 mm² FOV, 40 Hz (256 x 256 Pixels)
  • Large FOV: 0.25 mm² FOV, 15 Hz (256 x 256 Pixels)
• Multiple Interchangeable Objectives

Thorlabs' Miniature Two-Photon Imaging System (Mini2P) is a fast, high-resolution miniscope designed for in vivo two-photon imaging of non-stationary specimens. This Mini2P is based on the original design referenced in the paper by Zong. W., et al.** The lightweight, head-mounted design allows for stable calcium imaging without impediment to the sample. Interchangeable objectives facilitate use with cranial windows, prisms, and GRIN lenses for imaging tissue at different depths and orientations. The Mini2P allows for hundreds of neurons to be monitored in a single FOV, while visualization of up to a thousand cells is achievable through multiplane imaging controlled through the built-in microtunable lens.

A near-infrared, 920 nm femtosecond pulsed laser provides ideal excitation for two-photon imaging of GFP. Fiber coupling with a hollow-core photonic crystal fiber creates a stable system without complicated alignment procedures. Laser scanning is performed by a 2D microelectromechanical system (MEMS) device that is available for either fast scanning or large FOV applications. Signal detection is achieved with an on-board, dual-color silicon photomultiplier (SiPM) detector, eliminating the need for a fiber collection bundle that could impede movement. Laser scanning, signal detection, and z-scanning are all integrated into a flexible circuit controlled by a single miniaturized coax cable bundle and dedicated control box.

*The Mini2P microscope is under development and all specifications are subject to change.

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Flexible On-Board Electronics Drives Laser Scanning, Z-Focus and Collects Photons

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The Mini2P microscope is controlled via a fully integrated electronics control box.

**Large-Scale Two-Photon Calcium Imaging in Freely Moving Mice.

Zong, W., Obenhaus, H. A., Skytøen, E. R., Eneqvist, H., de Jong, N. L., ... & Moser, E. I.